RP-HPLC法测定AIDS合并肺部真菌感染患者伏立康唑的血药浓度

Determination of voriconazole in plasma from patients of AIDS complicated with pulmonary fungal infection by RP-HPLC

目的建立测定人血浆中伏立康唑浓度的RP-HPLC法,并测定AIDS合并肺部真菌感染患者伏立康唑的血药浓度。方法留取患者稳态谷和稳态峰浓度时的血样,以岛津LC-20A高效液相色谱仪进行测定。色谱柱为Diamonsil C_(18)柱,流动相为0.01 mol·L^(-1)醋酸铵-乙腈(50:50,V/V),流速0.8 mL·min^(-1),紫外检测波长255 nm,内标为酮康唑,柱温40℃,进样量50μL。结果色谱峰分离良好,血浆内源性杂质无干扰,回归方程为y=8.400 2x+2.26×10^(-2)(r=0.999 9),血浆中伏立康唑在0.10～19.87 mg·L^(-1)内线性关系良好,日内和日间精密度(RSD)均<5%。结论本方法灵敏、准确、重现性好,适于伏立康唑血药浓度的测定。

AIM To develop a RP-HPLC method for the determination of voriconazole in human plasma and to apply this method for patients of AIDS complicated with pulmonary fungal infection. METHODS Blood samples were taken from patients of AIDS complicated with pulmonary fungal infection treated with voriconazole. The chromatographic separation was achieved on a reverse-phase column Diamonsil C18 using 0.01 mol. L- 1 ammonium acetate-acetonitrile （50：50, V/V ）as mobile phase. The flow rate was 0.8 mL.min-1 and the detective wavelength was 255 nm. Keteconazole was taken as internal standard. The column temperature was 40℃ and injection volume was 50 uL. RESULTS The linear response in the concentration range of 0.10- 19.87 mg. L-1 was obtained in plasma. The linear regression equation was y = 8. 400 2x ＋ 2.26 x 10-2（ r = 0.999 9）. Intra-day and inter-day RSDs were below 5% for all samples. CONCLUSION The method is sensitive, accurate, and has good reproducibility. It is suitable for the determination of voriconazole in human plasma.