脓毒症时肠道细菌易位发生部位的研究

The site of bacteria translocation induced by sepsis

目的:研究脓毒症时肠道细菌易位发生的部位。方法:将36只大鼠随机分对照组和脓毒症组,各组内随机分为空肠组、回肠组和结肠组。空肠组大鼠于Treitz韧带处空肠置管,距离Treitz韧带20cm处造口;回肠组于回盲部上20cm处肠道置管,末端回肠造口;结肠组行盲肠置管。各组分别于肠道置管口灌入绿色荧光标记的大肠杆菌和乳果糖/甘露醇,于术后行肠道通透性检测,24h后荧光显微镜下观察大鼠肠系膜淋巴结、肝、脾组织中标记细菌的数量,肠系膜淋巴结细菌培养,并在荧光显微镜下观察。结果:空肠组、回肠组和结肠组大鼠肠黏膜的通透性和肠系膜淋巴结荧光细菌计数存在差异,回肠组明显高于空肠组和结肠组。结论:脓毒症时,各肠段细菌易位程度不同,回肠内易位细菌数量最多。

Objective：To confirm the site of bacterial translocation induced by sepsis.Methods：Thirty-six male Sprague-Dawley（SD）rats weighing 200-250 g were randomly divided into two groups：control group（n=18） and sepsis group（n=18）,each group were randomly divided into jejunum,Ileum and Colon groups.Escherichia Coli （ E.coli）labled with green fluorescent protein（GFP）,lactulose and mannitol were injected into the intestinal tract through the tube.Intestinal permeability was assessed after the models were accomplished.The mesenteric lymph nodes（MLN）,liver and spleen were taken for frozen section and examined for translocation of GFP labled E.coli using fluorescence microscopy.MLN were aseptically harvested for bacteria culture on time of 24 hours after GFP labeled E.coli administration.Results：The bacteria labled with green fluorescent protein were detected in tissue specimen harvested from sepsis groups,but were not detected in the control groups.And the ileum was highest in the intestinal permeability.Conclusion：Sepsis can induce intestinal bacterial translocation in rats.And the site of bacteria translocation is more obvious in the ileum.