丙型肝炎病毒分片段抗体检测及优势抗原表位分析

Detection of HCV Fragmental Antibody and Analysis of Prominent Antigenic Epitope

目的检测丙型肝炎病毒(HCV)分片段抗体,并分析HCV优势抗原表位,为HCV抗原、抗体检测及疫苗研究提供依据。方法收集经ELISA法检测抗HCV阳性血清样品90份,使用总抗体检测试剂及抗HCV分片段试剂进行检测,并用荧光定量PCR对抗体弱阳性及阴性样品进行复核,分析不同片段出现的阳性率,从而分析HCV的优势抗原表位。结果 90份抗HCV阳性的样品经总抗体检测试剂检测,其中阳性78份(86.67%);90份抗HCV阳性的样品中,分片段试剂检测1个片段以上阳性样品59份(65.56%);其中C、NS3、NS4及NS5片段抗体阳性分别为56份(94.92%)、57份(96.61%)、42份(71.19%)及47份(79.66%),NS3及C片段抗体是HCV的优势抗体。结论 HCV-C及NS3表位是HCV的优势抗原表位;HCV分片段抗体与总抗体检测试剂之间阳性检出率存在一定差异。

Objective To detect HCV fragmental antibody,analyze the prominent antigenic epitope and provide a basis for the detection of antigen and antibody of HCV as well as development of hepatitis C vaccine.Methods Ninety serum specimens proved as anti-HCV positive by ELISA were collected and tested by total anti-HCV detection kit and HCV fragmental antibody detection kit respectively,of which those with weak positive or negative results were retested by fluorescent quantitative PCR.The positive rates of various antibody fragments were evaluated,based on which the prominent antigenic epitope of HCV was analyzed.Results Of the 90 anti-HCV positive specimens proved by ELISA,78（86.67%）were confirmed as positive by total anti-HCV detection kit and 59（65.56%）as positive for more than one fragment by HCV fragmental antibody detection kit.Antibody fragments C,NS3,NS4 and NS5 were detected in 56（94.92%）,57（96.61%）,42（71.19%）and 47（79.66%）specimens respectively,of which NS3 and C were the prominent antibody of HCV.Conclusion HCV-C and HCV-NS3 are the prominent antigenic epitopes of HCV,and the detection rate of positive specimens by HCV fragmental antibody detection kit showed a certain difference with that by HCV antibody detection kit.