GnRH Ⅱ与GnRH Ⅰ对子宫内膜异位症患者间质细胞分泌VEGF作用的比较(英文)

Effect of GnRH Ⅱ and GnRH Ⅰ on secretion of VEGF by eutopic and ectopic endometrial stromal cells of endometriosis patients

目的:测定GnRH Ⅱ与GnRH Ⅰ对子宫内膜异位症(EMs)患者离体培养子宫内膜间质细胞分泌血管内皮生长因子(VEGF)的影响,探讨GnRH Ⅱ对EMs患者可能的作用。方法:给予原代培养的EMs患者在位及异位子宫内膜间质细胞不同浓度的GnRH Ⅱ,GnRH Ⅰ类似物(戈舍瑞林,goserelin)处理,同时设对照组(不加GnRH),采用酶联免疫吸附法(ELⅠSA)测定培养液中VEGF浓度,并进行比较。结果:EMs患者离体培养的异位子宫内膜间质细胞经48 h培养,能分泌VEGF,分泌量与在位子宫内膜间质细胞的相近,两者比较差异无统计学意义(P>0.05)。不同浓度的GnRH Ⅱ对EMs患者离体培养的在位和异位子宫内膜间质细胞VEGF的分泌有明显的抑制作用,呈剂量依赖性(P<0.05),且较GnRH Ⅰ类似物(戈舍瑞林)的作用更强(P<0.05)。不同浓度的GnRH Ⅱ对EMs患者离体培养的异位子宫内膜间质细胞VEGF分泌的抑制作用明显强于在位(P<0.05)。结论:EMs患者的异位子宫内膜间质细胞具有分泌VEGF的功能,分泌量与在位子宫内膜的相近,这对EMs的形成和发展可能起重要作用。Gn-RH Ⅱ呈剂量依赖性地抑制异位内膜间质细胞分泌VEGF,其抑制作用明显强于在位,且GnRH Ⅱ明显强于GnRH Ⅰ,为寻找EMs抗血管形成方面的新药治疗提供了新的依据。

Objective To determine the effect of GnRH Ⅰ and GnRH Ⅱ on the secretion of VEGF by eutopic and ectopic endometrial stromal cells cultured in vitro,and to provide theoretical basis for exploring new treatments for endometriosis（EMs）.Methods Eutopic and ectopic endometrium stromal cells cultured in vitro were treated with different concentrations of GnRH Ⅱ and a GnRH Ⅰ（goserelin）,and a control group was not treated by GnRH Ⅱ and GnRH Ⅰ.Enzyme linked immunosorbent assay（ELⅠSA） was used to measure the content of vascular endothelial growth factor（VEGF） protein in the medium of the above 2 groups.Results（1）There was no difference in the VEGF protein secreted by eutopic and ectopic stromal cells in the medium after being cultured in vitro for 48 h（P〉0.05）.（2）10-10,10-8,and 10-6mol/L GnRH Ⅱ dose-dependently reduced VEGF protein secreted by endometrial stromal cells（P〈0.05）,and the inhibition effect was stronger than that of GnRH Ⅰ（P〈0.05）.（3）The inhibition effect of GnRH Ⅱ on VEGF in ectopic stromal cells was stronger than that of eutopic stromal cells（P〈0.05）.Conclusion（1）Ectopic stromal cells cultured in vitro can secrete VEGF,which has no difference from the eutopic stromal cells,and which may play an important role in the formation and development of EMs.（2）GnRH Ⅱ can dose-dependently reduce VEGF protein secreted by ectopic and eutopic endometrial stromal cells cultured in vitro,and the inhibition effect is stronger than that of GnRH Ⅰ,providing theoretical basis for exploring new treatments for EMs.