骨质疏松大鼠骨髓间充质干细胞的生物学特性

Biological features of bone marrow mesenchymal stem cells of osteoporosis rats

背景:随着近年来对骨质疏松机制研究的深入,研究者逐渐把研究重点放在了成骨细胞的来源骨髓间充质干细胞上。目的:对骨质疏松大鼠骨髓间充质干细胞进行分离和体外培养,观察骨质疏松模型大鼠骨髓间充质干细胞的生物学特性,分析骨质疏松的细胞病理学机制,以期为骨质疏松的防治提供一个有意义的药物靶标。方法:选用10月龄SD雌性大鼠去卵巢增龄3个月来复制骨质疏松模型,设假手术对照组。运用密度梯度离心法对两组大鼠骨髓间充质干细胞进行分离和体外培养,运用扫描电镜对培养的骨髓间充质干细胞进行形态学观察,并行生长曲线及贴壁率检测。结果与结论:骨质疏松大鼠的骨髓间充质干细胞增殖能力明显下降,与对照大鼠相比存在许多结构特征的差异。体外实验结果表明,骨质疏松大鼠骨髓间充质干细胞的体外增殖能力下降可能是骨质疏松的细胞病理学机制。

BACKGROUND：With deep research of osteoporosis mechanism,investigators mainly focused on bone marrow mesenchymal stem cells（BMSCs）,the source of osteoblasts.OBJECTIVE：BMSCs from osteoporosis rats were isolated and cultured in vitro.To observe biological features of BMSCs of rat models of osteoporosis,to analyze cytopathology mechanism of osteoporosis,and to provide a significant drug target for prevention and treatment of osteoporosis.METHODS：The animal model of osteoporosis was established by ovariectomied method in 10-month-old female Sprague-Dawley rats.Sham operation control group was established.BMSCs were isolated from the rats and cultured in vitro by density-gradient centrifugation in both groups.Morphology of BMSCs was observed under a scanning electron microscope.The growth curve and adhesion rate were measured.RESULTS AND CONCLUSION：Reproductive activity of BMSCs of osteoporosis rats was significantly decreased,and there were many differences in structural features compared with control rats.In vitro experiment results demonstrated that the decrease in reproductive activity in vitro of BMSCs of osteoporosis rats contributes to osteoporotic cytopathology.