自体骨髓动员对急性缺血心肌血管再生的影响

Effects of autologous bone marrow mobilization on vascular regeneration of acute ischemic myocardium

背景:动物实验表明,应用干细胞移植有可能取代受损心肌细胞并建立新的血管来改善血液供应。目的:观察应用集落刺激因子骨髓动员急性心肌梗死模型猪心功能及新生血管的作用。方法:健康太湖梅山猪10头,建立猪心肌梗死模型后,随机分为2组。对照组4周后经冠状动脉注射DMEM;实验组心肌梗死后3h并连续5d注射粒细胞集落刺激因子。心肌梗死后第8周行超声心动检测心功能,检测不同时间点血清血管内皮生长因子和转化生长因子?值,梗死区标本苏木精-伊红染色观察病理变化及Ⅷ因子免疫组织化学染色检测毛细血管密度。结果与结论:骨髓动员后实验组血清血管内皮生长因子,转化生长因子?水平上升明显,标本Ⅷ因子免疫组织化学染色法血管密度有明显上升。结果提示集落刺激因子诱导的自体骨髓干细胞动员对急性缺血心肌血管再生有很明显的促进作用。

BACKGROUND：Animal trials have suggested that stem cell transplantation can replace damaged myocardial cells and establish new vessels for improving blood supply.OBJECTIVE：To study the effect of autologous bone marrow mobilization using colony stimulating factor on cardiac function and new vessels in swines with acute myocardial infarction.METHODS：A total of 10 healthy Taihu Meishan swines were divided into two groups following model establishment of myocardial infarction.In the control group,the swines were injected with DMEM through coronary artery at 4 weeks later.In the experimental group,the swines were infused with granulocyte colony-stimulating factor at 3 hours following myocardial infarction for 5 consecutive days.Cardiac function was examined using ultrasonic cardiograph at 8 weeks following myocardial infarction.Expression of serum vascular endothelial growth factor and transforming growth factor beta（TGF-？） was detected at various time points.Hematoxylin-eosin staining was used to observe pathological changes in infarct region.Capillary density was determined using immunohistochemical staining for Ⅷ factor.RESULTS AND CONCLUSION：Following mobilization,serum vascular endothelial growth factor and TGF-？ expression were significantly increased in the experimental group.Immunohistochemical staining for Ⅷ factor showed that vascular density was significantly increased.These indicated that colony stimulating factor-induced autologous bone marrow stem cell mobilization can obviously promote vascular regeneration of acute ischemic myocardium.