聚四氟乙烯膜表面改性后成骨细胞相容性研究

Biocompatibility of PTFE membrane by oxygen plasma immersion ion implantation

目的:评价O2-PⅢ表面处理聚四氟乙烯(PTFE)膜后,其上培养生长的成骨细胞生物学相容性变化。方法:O2等离子体浸入离子植入技术(plasma immersion ion implantation,PⅢ)处理PTFE膜表面,进行膜表面改性;O2-Ⅲ处理组和未处理组的PTFE膜分别通过扫描电镜(SEM)观察表面形态、表面接触角测定分析其亲水性;分别在2组膜上接种成骨细胞,6h后SEM观察成骨细胞贴附情况,并分别于6、24、48h后4',6二脒基-2-苯吲哚盐酸(DAPI)核染色,荧光显微镜下观察成骨细胞的数量,进一步评估2种膜表面对成骨细胞生物相容性的影响。结果:O2-PIII处理改变了PTFE膜表面形态和增强了其亲水性。2组样本表面接种成骨细胞后SEM及荧光照片显示:O2-PⅢ处理组相对于未处理组更利于成骨细胞的吸附和增殖。结论:O2-PⅢ处理后可提高PTFE膜表面成骨细胞的相容性,可望在临床实际应用中促进组织的修复和再生。

Objective：To investigate the biocompatibility of polytetrafluoroethylene（PTFE）membrane after oxygen plasma immersion ion implantation（O2-PIII）.Methods：O2-PIII was conducted to modify the PTFE surface.Both the treated and control specimens were characterized by scanning electron microscopy（SEM）and contact angle measurement.Osteoblasts were cultured on PTEE surface.After different culturing times（6,24,48 h）,the samples seeded with osteoblasts were stained with 6-diamidino-2-phenylindole dihydrochloride hydrate（DAPI）.The stained cells were viewed and photographed with a fluorescence microscope.After 6h culture,the morphology of the cells on the specimens was also investigated by SEM.Results：The O2-PIII treatment improved the hydrophilicity and roughness of PTFE membrane significantly.Cell adhesion and proliferation in the O2-PIII group were better than those in the control group.Conclusion：The biocompatibility of PTFE membrane is enhanced after O2-PIII treatment.