维生素E在青年和老年大鼠肾脏缺血/再灌注损伤中的作用

The effect of vitamin E on renal ischemia/reperfusion injury in young and aged rats

目的:探讨维生素E(VE)在青年和老年大鼠肾缺血/再灌注损伤(RI/RI)中的作用。方法:采用夹闭双侧肾动、静脉45min后恢复血流的方法制作RI/RI模型,测定血清中尿素氮(BUN)、肌酐(Scr)、丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮(NO)、诱生型一氧化氮合酶(iNOS)浓度,免疫组化检测肾皮质热休克蛋白70(HSP70)表达。流式细胞术检测肾皮质细胞凋亡率。结果:缺血/再灌注(I/R)后BUN、Scr含量明显升高,老年I/R组MDA含量高于青年I/R组,SOD含量低于青年IR组,HSP70、NO以及肾皮质细胞凋亡率高于control组;VE可显著降低RI/RI大鼠BUN、Scr、MDA、iNOS水平,升高NO和SOD水平,增加HSP70的表达,降低肾皮质细胞凋亡率。结论:VE可通过促进肾组织HSP70的表达,增加NO和SOD水平,提高大鼠体内清除自由基的能力,从而对青、老年大鼠肾缺血/再灌注损伤(RI/RI)起到一定的保护作用。

Objective：To investigate the effect of vitamin E on renal ischemia/reperfusion injury（RI/RI） in young and aged rats.Me-thods：The model of（RI/RI）was induced by bilateral clamping the renal artery and vein for 45 min followed by reperfusion.The contents of BUN,Scr,MDA,SOD,NO and iNOS were measured.Proteins of HSP70 were observed by immunohistochemistry.Flow cytometer was used to estimate the apoptosis rate of renal cortex cells.Results：After ischemia/reperfusion injury,the contents of BUN,Scr and iNOS were increased.Compared with young ischemia/reperfusion group,the contents of MDA were higher and the contents of SOD were lower in aged ischemia/reperfusion group.The expression of HSP70,the contents of NO and the apoptosis rate of renal cortex cells were higher in aged ischemia/reperfusion group than that in control group.Vitamin E significantly decreased the contents of BUN,Scr,MDA and iNOS,and increased the contents of NO and SOD after RI/RI.The expression of HSP70 was increased in both VE groups than that in both I/R groups.The apoptosis rate of renal cortex cells was less in both VE groups than that in both I/R groups.Conclusion：Vitamins E may up-regulate the expression of HSP70,increase the contents of SOD and NO,and enhance an ability of clear free radicals,which may contributes to decreasing renal ischemia/reperfusion injury in young and aged rats.