抗bFGF抗体对肺纤维化模型的干预作用

The interventional effect of anti-basic fibroblast growth factor on the model of bleomycin-induced pulmonary fibrosis in rats

目的:研究抗bFGF抗体对大鼠肺纤维化模型的干预作用及可能的机制。方法:雌性Wistar大鼠30只,体重180～250g,按照随机数字表法将大鼠随机分为3组(n=10):①对照组(C组);②模型组(M组);③抗bFGF抗体组(K组)。M、K组给大鼠气管内注入博莱霉素复制肺纤维化模型,C组气管内注入同等剂量的生理盐水作对照,K组于造模后1,2,3,8,12,19,25d腹腔内注射抗bFGF抗体。上述各组均于注药后1、4周各宰杀5只。通过苏木素-伊红染色观察肺泡炎、Masson胶原染色观察肺纤维化、用免疫组化及酶联免疫吸附测定(ELISA)法检测bFGF蛋白在大鼠肺组织,血清及肺泡灌洗液(BALF)的表达。结果:在1、4周时M组与C组比较肺泡炎、肺纤维化程度明显,M组在肺组织、血清和的BALF中的bFGF表达与C组的bFGF表达比较升高;在1、4周时K组与M组比较肺泡炎、肺纤维化程度减轻,K组在肺组织、血清和的BALF中的bFGF表达与M组的bFGF表达比较均有降低。结论:抗bFGF抗体可减轻博莱霉素诱导的肺纤维化,其抗纤维化作用的机制与抑制bFGF表达有关。

Objective：To study the effect of anti-basic jibroblast grouth factor（bFGF） on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism.Methods：Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin（model group,group M）.Then the rats received anti-bFGF on 1,2,3,8,12,19,25 days intracavitary injection（group K）,normal saline（group C） orally.Normal controls received normal saline both intratracheally and orally.Five rats in each group were sacrificed on 1,4 week after intratracheal instillation.Histological changes of the lungs were evaluated by HE stain and Massons trichrome stain.Lung expression of bFGF proteins was assessed by immunohistochemistry and the level of bFGF protein in serum and BALF was further measured by ELISA.Results：Pulmonary fibrosis of group M was higher than that of group C.bFGF in group M was higher than that in group C in lung,serum and BALF on 1,4 week.Pulmonary fibrosis of group K was lower than that of group M.bFGF in group K was lower than that in group M in lung,serum and BALF on 1,4 week.Conclusion：Anti-bFGF alleviates bleomycin-induced pulmonary fibrosis in rats.Inhibiting the expressions of bFGF in lung tissues may be one of the mechanisms.