烟草下脚料发酵制取乙醇

Ethanol produced by fermentation of waste tobacco materials

通过单因素实验考察了硫酸浓度、固液比和水解时间对硫酸水解的影响。结果显示最优条件为:硫酸浓度为50%(w/w),固液比为10%(w/v),时间为100 min。烟草下脚料在最佳硫酸水解条件下,经5倍稀释,中和pH值至5～6。取经过滤后的水解液(FH)用酿酒酵母(Sacchharomyces cerevisiae)发酵产生乙醇,最大的乙醇浓度和乙醇产量分别为1.09g/L和54.5 g/kg。未过滤水解液(UFH,包括水解残渣)加入纤维素酶(70 U/100 mL)和酿酒酵母(Sacchharomyces cerevisiae)进行发酵,最大的乙醇浓度和乙醇产量分别为1.23 g/L和61.5 g/kg。

The potential of tobacco waste as a raw material for ethanol production was investigated.In this work,the effects of concentration of sulfuric acid,solid-liquid ratio and time on reducing sugar yield were studied using single factor tests.Best results were achieved at 50%（w/w） acid loading,10%（w/v） solid-liquid ratio and 100 min.Under the optimal hydrolysis conditions,the maximal ethanol concentration and ethanol yield,with Sacchharomyces cerevisiae,were 1.09 g/L and 54.5 g/kg,respectively from filtered hydrolysate（diluting 5 times）.However,the maximal ethanol concentration and ethanol yield,with Sacchharomyces cerevisiae,were 1.23 g/L and 61.5 g/kg,respectively from unfiltered hydrolysate（adding 70 U/100 mL cellulose and diluting 5 times）.