高效液相色谱法检测鸡蛋中的三聚氰胺

Determination of melamine in eggs by high performance liquid chromatography

建立了高效液相色谱法检测鸡蛋中三聚氰胺的方法。样品用1%三氯乙酸和2.2%乙酸铅超声提取。色谱柱为HypersilODS100×4.6mm,5μm;流动相为乙腈:辛烷磺酸钠离子对试剂(pH=3)=15:85(V/V);流速为1mL/min;检测波长为210nm;柱温为40℃。三聚氰胺在0.2μg/mL～10μg/mL范围内,有良好的线性关系。在空白样品中添加0.2μg/mL、0.4μg/mL、1.0μg/mL三个质量浓度的标准工作液,回收率在82.0%～104.0%,相对标准偏差为6.81%(n=9)。

A method was reported for the determination of melamine in eggs.After being extracted by 1%Trichloroacetic acid solution and 2.2% lead acetate from samples,melamine was separated on a Hypersil ODS 100×4.6mm,5μm C_18 column with a volume ratio of acetonitrile and sulfonic sodium octane（pH=3） of 15：85,and was detected by an ultraviolet detector at a wavelength of 210nm.The temperature of column was 40℃.The linear ranges were from 0.2μg/mL to 10.0μg/mL.The results indicated that the average recoveries were 82.0%～104.0% for high,middle and low concentration levels by this method.The relative standard deviations RSD was 6.81%（n=9）.