肝素在大鼠外伤性鼓膜穿孔愈合中对转化生长因子-α的影响

The effect of heparin on the TGF-α during the traumatic tympanic membrane perforation in animal models

目的:探讨局部应用肝素在外伤性鼓膜穿孔愈合中对转化生长因子-α(TGF-α)的影响。方法:建立大鼠鼓膜穿孔模型50只,将其随机分成两组,分别用肝素明胶海绵、表皮生长因子(epidermial grouth factor,EGF)明胶海绵黏补,空白对照,观察不同时间点不同组鼓膜愈合情况并取其标本进行免疫组织化学染色,测其转化生长因子-α(TGF-α)的表达水平。结果:肝素组与空白组TGF-α表达有差异(P<0.05),肝素组与EGF组表达基本无差异(P>0.05)。结论:局部应用肝素能促进转化生长因子-α(TGF-α)在鼓膜各层的表达,并延长其表达时间。

Objective： To approach the efficacy of local application heparin on the TGF-α during the treatment of acute traumatic tympanic membrane perforation （TMP） in the animal models. Methods： Developed an animal model of traumatic TMP in the rat and 50 wistar rats were used.They were randomly divided into two groups and treated by heparin combined with gelfoam pledger, EGF combined with gelfoam pledger which was placed over the TMP and blank control separately, then observe the state of the TMP and examined by immunohistochemical method using polyelonal expression level of TGF-α. Results： Statistical difference between treated and control ears in the expression of TGF-α was evident（P〈0.05）. Statistical difference between heparin and EGF ears in the expression of TGF-ot was not evident（P〉0.05）. Conclusion： Local application heparin can accelerate the expression of healing of TGF-α in acute traumatic TMP in the animal models.