黑龙江省野猪线粒体D-Loop区的生物信息学分析

Bioinformatic Analysis of Heilongjiang Wild Boar Mitochondria D-Loop

对Genbank中野猪的线粒体D-Loop区的基因序列进行了SNP分析及分子进化树构建。结果表明:黑龙江野猪线粒体DNA的D-Loop区全长1 145 bp,A+T含量(61.3%)明显高于G+C含量(38.7%);发现了93个多态位点,界定了20种单倍型,野猪mtDNA D-Loop区核苷酸多样性为0.02553,单倍型多样性为0.995,平均核苷酸差异数为15.443;黑龙江野猪与意大利和瑞典野猪亲缘关系最近,与马来西亚野猪的亲缘关系最远,推测黑龙江野猪含有欧洲野猪血统。

All of the mitochondrial DNA D-Loop sequences in Genbank currently were analyzed by some tools of bioinformatics.The results showed that,wild boar D-Loop was 1 145 bp,A＋T content（61.3%）was higher than（G＋C）38.7%,93 SNP and 20 haplotypes were found.The nucleotide diversity of mtDNA D-Loop was 0.02553,the haplotype gene diversity was 0.995,and the average number of nucleotide differences was 15.443.The phylogenetic tree showed that Heilongjiang wild boar was closest to Italian and Sweden wild boar,but farthest to Malaysia wild boar,presumed that Heilongjiang wild boar including Europe wild boar blood.