草鱼鱼鳞中提取卵磷脂的最佳工艺研究

The optimum conditions forextraction of lecithin from the fish-scale of grass carp

提取草鱼鱼鳞中卵磷脂,研究提取时间、乙醇浓度及料液比等对卵磷脂提取率的影响,对草鱼鱼鳞中卵磷脂的提取工艺条件进行初探。以草鱼鱼鳞为原料,乙醇溶液为萃取剂,提取卵磷脂。提取时间、乙醇浓度及料液比对卵磷脂提取率均有影响,由正交试验结果分析发现乙醇浓度是影响卵磷脂提取率的主要因素。通过正交试验,确定了提取卵磷脂的最佳工艺条件:提取温度为25℃,提取时间为1 h,料液比为1∶20(mL/g),乙醇浓度为95%。

This paper deals with extracting lecithin from the fish-scale of grass carp and determining its optimum conditions.Lecithin from the fish-scale of grass carp was extracted using ethanol.The effects of time,temperature,the amount and concentration of ethanol on extraction rate were studied.The results showed that time,temperature,the amount and concentration of ethanol all affect extraction rate.It is found out that concentration of ethanol is the main factor by means of orthogonal design.The optimum conditions that lecithin is extracted with ethanolare： 25℃;1 h（extraction time）;weight of fish-scale： volume of ethanol =1∶20（mL/g）;95% ethanol.