穿心莲抗人巨细胞病毒的体外实验研究

Anti-cytomegalovirus Effect of Andrographis Paniculata in Vitro

目的研究穿心莲体外抗人巨细胞病毒(HCMV)的效果和安全性。方法用细胞病变法和噻唑蓝(MTT)法检测穿心莲抗HCMV的最大无毒浓度、最小有效浓度和治疗指数,并与更昔洛韦、金银花、鱼腥草、大青叶进行比较。结果更昔洛韦、金银花、鱼腥草、大青叶及穿心莲的最大无毒浓度分别为10,3000,30000,30000,3000μg·mL-1,最小有效浓度分别为10,3000,3000,3000,300μg·mL-1,治疗指数分别为1,1,10,10,10。结论穿心莲具有体外抗HCMV效果,其抗病毒作用高于金银花、鱼腥草、大青叶;细胞毒性与金银花相同,略高于鱼腥草、大青叶。其治疗指数高于金银花,与鱼腥草、大青叶相同,具有潜在的临床应用前景,值得进一步深入研究。

Objective To study the in vitro anti-cytomegalovirus effect and the security of andrographis paniculata. Methods The 0% toxic dose （TD0）,minimum effective concentration （MEC） and therapeutic index （TI） of anti-cytomegalovirus activity by andrographis paniculata were detected with cytopathic assay and MTT method,and compared with those of ganciclovir,honeysuckle,houttuynia cordata and folium isatidis. Results The TD0 of ganciclovir,honeysuckle,houttuynia cordata,folium Isatidis and andrographis paniculata were 10,3 000,30 000,30 000,3 000 μg·mL-1,the MEC were 10,3 000,3 000,3 000,300 μg·mL-1,TI were 1,1,10,10 and 10,respectively. Conclusions Andrographis paniculata showed a good in vitro anti- cytomegalovirus effect,which is higher than honeysuckle,houttuynia cordata and folium isatidis,and a equivalent cytotoxicity to honeysuckle but higher than houttuynia cordata and folium isatidis. The TI of andrographis paniculata is higher than honeysuckle but equivalent to houttuynia cordata and folium isatidis. Therefore,andrographis paniculata has the potential advantages for clinical use and worth further study.