气相色谱法测定一次性使用输液器中丁酮与环己酮残留量

Determination of the Residual Solvent of Butanone and Cyclohexanone in Infusion Sets for Single Use by GC

目的建立气相色谱法测定一次性使用输液器中粘合剂丁酮及环己酮残留量。方法采用DB-FFAP毛细管柱(30m×0.32mm,0.5μm),FID检测器,载气为氮气,柱温60℃,以20℃·min-1升温至120℃保持3min,进样口温度170℃,检测器温度250℃,分流比1:1,采用内标法定量。结果丁酮回归方程为:Y=0.0231X-0.0408,线性范围为5.06～101.18μg·mL-1,r=0.9989(n=7),平均加样回收率为98.93%(RSD=4.30%,n=9),环己酮回归方程为:Y=0.0303X-0.0289,线性范围为4.88～97.50μg·mL-1,r=0.9995(n=7),平均加样回收率为104.52%(RSD=2.20%,n=9)。结论该方法简便快捷,能准确有效地测定丁酮及环己酮的残留量。

Objective To establish a method for content determination of the residual solvent of butanone and cyclohexanone in infusion sets for single use by GC. Methods The analysis was performed on capillary column of DB-FFAP（30 m×0.32 mm,0.5 μm） ,with FID detector,using nitrogen as carrier gas. The column temperature was initially 60 ℃,increased at a rate of 20 ℃·min-1 to 120 ℃ and maintained at 120 ℃ for 3 min. The injector temperature was set at 170 ℃ and the detector temperature was 250 ℃. The split ratio was 1：1. The residual solvents were calculated by internal standard method. Results The standard curve of butanone was Y=0.023 1X-0.040 8,within the range of 5.06-101.18 μg·mL-1,r=0.998 9（n=7）.The average recovery of butanone was 98.93%（RSD=4.30%,n=9）. The standard curve of cyclohexanone was Y=0.030 3X-0.028 9,within the range of 4.88-97.50 μg·mL-1,r=0.999 5（n=7）.The average recovery of butanone was 104.52%（RSD=2.20%,n=9）. Conclusion The method is simple and rapid,which can be used for detection of the residual solvents of butanone and cyclohexanone accurately.