雌二醇对人类成骨细胞HOS TE85增殖及Ⅰ型胶原产生的影响

Effects of Estradiol in Proliferation and Type I collagen Synthesis in Human Osteoblast-like Cells TE85

雌激素替代疗法对更年期骨质疏松的预防及治疗效果显著，但作用机理不很明确，本研究通过体外培养的人类成骨细胞ＨＯＳＴＥ８５，观察雌二醇对成骨细胞增殖及Ⅰ型胶原产生的影响。方法：在体外培养的人类成骨细胞ＨＯＳＴＥ８５培养液中，加入雌二醇（０．０１－１０ｎｍｏｌ／Ｌ），分别培养２４、４８、７２及９６ｈ，于结束培养前６ｈ，加入３Ｈ－胸腺脱氧核苷检测细胞增殖；或于细胞开始培养时，加入３Ｈ－脯氨酸检测Ｉ型胶原产生。结果：在２４ｈ时，雌二醇刺激细胞增殖，但与对照组无显著差异；在４８、７２及９６ｈ时，雌二醇抑制细胞增殖。但无明显量效关系。在４８、７２ｈ时，雌二醇明显刺激细胞Ｉ型胶原产生，量效关系显著，在９６ｈ时。也能刺激Ｉ型胜原产生。结论：雌二醇对人类成骨细胞ＨＯＳＴＥ８５增殖具有短期刺激，长期抑制的作用，对Ｉ型胶原产生具有促进作用，这可能是其临床治疗作用的主要机制。

Objective:Estrogens play an important role the prevention and treatment of Postmenopausalosteoporosis.Whereas the pharmacological mechanisms of estrogen action on bone are poorlyunderatood.The present study was designed to investigate the effects of 17 -estradiol(E2)on cellproliferation and type I collagen synthesis in human osteoblast-like cell TE85(HOS TE85)Methods:TOS TE85 cells were incubated with E2 (0.01010nmol/L in 48 wells for 24,48,72and 96 hours,respectively,then 3H-thymidine was added to each well for 6 h before completion of the assay ofproliferation,or 3H-proline was added to each well at the beginning of the experiment for themeasurement of type I collagen synthesis.Results:The proliferation of cells was slightly stimulated byE2 in 24h, whereas that of celle was inhibited by E2 in 48,72and 96h.E2 promoted type I collagensynthesis in 48,72and 96h.Conclusion:For the cultured osteoblasts,E2 has both stimulating andinhibiting effects on proliferation as time progresses,while has steady promoting effect on type Icollagen synthesis.The observed effects of E2 on osteoblasts may account for the clinical treatment of postmenopausal osteoporosis.