液体法与固体培养法检测解尿脲原体和人型支原体的比较

Comparison the use of liquid culture medium and solid culture medium in detection Ureaplasma urealyticum and Mycoplasma hominis

目的:比较肉汤培养法与A7固体琼脂平板法平行检测人型支原体和解尿脲原体结果的一致性。方法收集门诊病人泌尿生殖道标本60例,分别用肉汤培养法和A7固体琼脂平板法及金标准方法(10B肉汤和A8琼脂平板)检测,并对检测结果进行统计分析。结果:60例标本中,金标准方法检测出阳性19例;肉汤培养阳性25例,敏感性100%(19/19),特异性为85.4%(35/41);固体培养阳性16例,敏感性84.2%(16/19),特异性100%(41/41)。肉汤培养法与标准方法结果有显著性差异(P(0.05),固体培养法与标准方法无显著性差异(P>0.05)。结论:固体培养法比肉汤培养法更适于临床支原体检测,但还需要进一步改进。

Objective： To compare the outcomes of detecting Ureaplasma urealyticum and Mycoplasma hominis with broth culture method and the agar A7 plate method.Methods： A total of 60 samples collected from urogenital tract were cultured with broth culture method,agar A7 plate method,and golded standard method（10B bouillon and A8 agar medium） for detection of Ureaplasma urealyticum and Mycoplasma hominis.Results： Of the 60 samples,19,25,and 16 were revealed as positive according to the golden standard method,broth culture method and agar A7 plate method,respectively.The sensitivity rate specificity rate was 100%（19/19） 85.4%（35/41） for the broth culture method,and 84.2%（16/19） 100%（41/41） for the agar A7 plate method.There was significant difference in the sensitivity or specificity between the broth culture and golden standard method,while no significant difference between agar A7 medium and the golden standard method.Conclusion： Agar A7 medium is more applicable in clinical work for the detection of mycoplasma compared to broth culture medium,but it needs further improvements.