黄椒草的组织培养

Tissue Culture ofCryptocoryne Affinis

采用不同的消毒方法与不同的培养基配方对黄椒草进行组培快繁试验,结果表明,取幼胚作外植体,用75%的酒精浸30s+0.05%升汞浸15min+无菌水冲洗5次进行消毒;消毒后外植体导入MS+椰子水40g/L+蔗糖10g/L+活性炭1g/L+琼脂粉5g/L诱导丛生芽,用MS+NAA0.1mg/L+IBA0.2mg/L+椰子水40g/L+蔗糖20g/L+活性炭2g/L+琼脂粉4g/L培养壮苗、诱导生根效果较好。组培快繁的黄椒草苗顺利通过旱培移栽、转水等驯化,批量投入生产。

Different ways of sterilization and medium formula were used for tissue culture and rapid propagation of Crytocoryne Affinis. The results showed that good effect obtained by using young germ as explant soaking in 75% ethanol and 0.05% mercuric chloride for 30s and 15mins respectively, and washed with aseptic water for 5 times. The appropriate medium for bud growth is MS＋coconut juice 40 g/L＋sucrose 10 g/L＋ activated charcoal.1 g/L＋agar powder 5 g/L. Medium with MS ＋NAA0.1 mg/L＋ IBA0.2 mg/L＋coconut juice 40 g/L＋ sucrose 20 g/L＋ activated charcoal2 g/L＋agar powder 4 g/L is good for healthy seedling and roots development. Crytocoryne Affinis seedling by tissue culture and rapid propagation were transplanted, water growth acclimated and quantity produced smoothly.