不同规模猪场种猪猪瘟抗体检测与猪瘟净化

Eradication and immunity evaluation on classic swine fever for pig farms of different sizes

采用HerdChek猪瘟抗体检测试剂盒检测福建省10个不同规模养猪场的种猪血清抗体,评价种猪群猪瘟免疫状况。结果表明:10个猪场种猪群猪瘟抗体平均合格率(抗体阻断率≥50%)为88.11%,猪瘟抗体平均不合格率(抗体阻断率<50%)为11.89%。随后对猪瘟抗体不合格的种猪注射猪瘟活疫苗加强免疫,28d后再次检测猪瘟抗体,10个猪场种猪群猪瘟抗体平均合格率达到91.44%,比首次检测提高了3.33个百分点,结合2次猪瘟抗体检测结果,淘汰加强免疫后猪瘟抗体不合格的种猪;并初步探讨了以抗体检测为基础的猪瘟净化措施。

To evaluate the status of the breeding pigs＇ immunity against classic swine fever virus （CSFV）,serum samples from 10 pig farms of different sizes in Fujian were collected and tested with HerdChek CSFV-ELISA kit. The results showed that the average passing rate （i.e.,antibody blocking rate≥50%） of the pigs with sufficient antibody against CSFV on the 10 farms was 88.11%. The average unqualified （i.e.,blocking rate50%） rate was 11.89%,ranging from 5.36% to 28.63%. A booster vaccination with active vaccine was implemented on the breeding swine that failed the test. Four weeks after the vaccination,a similar test was conducted again. It showed the average passing rate had reached 91.44%,i.e.,an increase of 3.33% over the rate prior to the booster vaccination. Based on the results,some preliminary measures for the CSFV eradication program is presented.