大鼠全脑缺血再灌注损伤后神经元凋亡及认知功能的研究

The research of cognitive and neural cell apoptosis in rats with cerebral ischemia reperfusion injuy

目的探讨大鼠脑缺血再灌注海马神经元凋亡及认知功能变化。方法雄性SD大鼠60只,随机分成假手术组(SH组)、模型组(IR组)。模型组采用4-VO法建立SD大鼠全脑缺血模型,不同时间点进行水迷宫测试。TUNEL法检测海马CA1区凋亡细胞。结果尼氏染色显示,与假手术组间比较,缺血组海马CA1区神经元数量显著下降(P<0.01);TUNEL法检测显示:与假手术组相比,凋亡指数显著增加(P<0.01)。Morris水迷宫检测,与假手术组相比,模型组大鼠学习阶段寻台时间明显延长,而其记忆测试潜伏期也明显长于假手术组。结论大鼠全脑缺血再灌注损伤后神经元凋亡显著增加,认知功能明显下降。

Objective To study the cognitive and neural cell apoptosis in rats withal ischemia reperfusion injury.Methods 60 male Sprague-Dawley rats were randomly divided into Sham-operated group（SH）and model group（IR）.The rats of model groups were induced by four-vessel occlusion（4VO）.At different times after injury,water maze was employed and neuronal apoptosis was detected by TUNEL staining and analyzed by computer image analysis system.Results the nissl staining showed that the number of hippocampus CA1 neurons in model group was lower than Sham-operated group（P0.01）.The results of TUNEL method showed that the number of apoptotic cells in model group was more.In Morris water maze tests,the incubation period of seeking the platform and the learning and memorizing ability in model group were longer than sham-operated group.Conclusion sThe number of hippocampus neurons was increased significantly after cerebral ischemia reperfusion injury in rats and the cognitive ability was decreased.