胃癌中PDGF-B mRNA与蛋白的表达及其临床病理学意义

PDGF-B mRNA and protein expression in gastric carcinoma tissues and their clinicopathological significance

目的:研究PDGF-B基因与胃癌发生、发展的关系。方法:采用逆转录PCR与免疫组化方法(S-P法),对40例中晚期胃癌、20例早期胃癌和20例正常胃黏膜组织的PDGF-BmRNA与PDGF-B蛋白进行检测。结果:PDGF-BmRNA与PDGF-B蛋白在中晚期胃癌表达率分别为87.5%(35/40)、77.5%(31/40),在早期胃癌表达率分别为80.0%(16/20)、70.0%(14/20),明显高于正常胃黏膜组织表达率10.0%(2/20)、10.0%(2/20)(P<0.01);PDGF-BmRNA表达与肉眼类型、分化程度、淋巴结转移密切有关(P<0.05);PDGF-B蛋白表达与肉眼类型、分化程度密切有关(P<0.05)。结论:PDGF-BmRNA、PDGF-B蛋白在胃癌组织中高表达,并与胃癌的发生、发展,侵袭及淋巴结转移密切关系,可作为判断胃癌生物学行为和预后评估的重要指标。

Objective：To explore the relationship of PDGF-B gene and carcinogenicity and progression of gastric cancer.Methods：Immunohistochemical technique （S-P method） and RT-PCR were used to detect the PDGF-B gene（PDGF-B mRNA） and PDGF-B protein in 40 samples of advanced,20 of early gastric cancer and another 20 samples of normal gastric mucosal tissues,respectively.Results：PDGF-B mRNA and PDGF-B protein were positively expressed respectively in 87.5%（35/40） and 77.5%（31/40） of the advanced tissues,with a corresponding expression of 80.0%（16/20） and 70.0%（14/20） in the early gastric cases,which were significantly higher as compared with the normal gastric mucosal tissues against 10.0%（2/20） and 10.0%（2/20）（ P0.01）.Apart from that,PDGF-B mRNA expression was closely correlated with gross findings,degree of differentiation and lymph node metastasis,but PDGF-B protein expression was associated with gross findings and differentiation extent （P0.05）.Conclusion：PDGF-B mRNA and PDGF-B protein were found highly expressed in gastric tumor tissues and to be strongly associated with the development,progression,invasion and lymph node metastasis,suggesting that they may serve as important indexes in estimating the biological behavior and prognosis of gastric carcinomas.