摘要
目的研究散发性戊型肝炎(HE)患者病毒血症、粪便排病毒及抗体消长规律。方法采用逆转录套式聚合酶链反应(RTnestedPCR)动态检测血清和粪便HEVRNA,采用HEVORF2、ORF3合成多肽单独和联合EIA法动态检测血清抗HEV。结果血清HEVRNA阳性患者占71%(44/62),平均持续时间为病后20.6天(全程随访32例);粪便HEVRNA阳性标本中有96.2%(25/26)出现在病后20天内;血清抗HEVIgM和IgG阳性率于发病半月内分别为71.1%(32/45)和97.8%(44/45),随病程的延长IgM较早阴转。结论病毒血症和粪便排病毒主要出现在急性早期;联合检测ORF2和ORF3抗体可提高EIA试验的敏感性。
Objective To study the viremia, fecal shedding of HEV and dynamics of anti HEV in the patients with sporadic hepatitis E.Methods HEV RNA was detected in serial samples of sera and feces by reverse transcription nested polymerase chain reaction (RT nested PCR). Anti HEV was detected in serial sera by enzyme linked immunosorbent assay (EIA) based on two synthetic peptides from ORF2 and ORF3 of HEV genome.Results Viremia of HEV RNA was detected in 71% (44/ 62) of patients, persisted for 20.6 days after the onset of disease on an average. 96.2% (25/ 26) of fecal specimens with HEV RNA were detected within 20 days after the onset of disease. The positive rates of anti HEV IgM and IgG with in 15 days after the onset of disease were respectively 71.1% (32/ 45) and 97.8% (44/ 45), which decreased more dramatically in IgM than in IgG with the course of the disease. Conclusion Viremia and fecal shedding are common during early acute phase of the disease. The sensitivity of EIA using both ORF2 and ORF3 peptides is higher than that of either alone. As a diagnostic marker of hepatitis E, Anti HEV IgM seems to be more specific and anti HEV IgG seems to be more sensitive.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第1期7-10,共4页
Chinese Journal of Infectious Diseases
基金
浙江省自然科学基金
浙江省教委及浙江省卫生厅资助
关键词
戊型肝炎
散发性
病原学
血清学
PCR
ELISA
Hepatitis E virus Open reading frame Polymerase chain reaction Enzyme linked immunosorbent assay