608例早孕绒毛宫内感染巨细胞病毒的免疫组织化学诊断

Study on immunohistochemistry diagnosis of CMV infection in 608 cases of villi at first trimester of pregnancy

目的建立简便、快速的巨细胞病毒（ＣＭＶ）胚胎感染的诊断方法，早期诊断胚胎ＣＭＶ感染。方法应用免疫组化过氧化物酶抗过氧化物酶（ＰＡＰ）方法，检测与观察早孕绒毛组织的ＣＭＶ感染病灶。结果早孕绒毛６０８例，感染ＣＭＶ２２２例（３６．５％）。感染灶可分为三类，点状感染占７１％（１５８／２２２），巢状感染占１７％（３７／２２２），弥散状态感染占１２％（２７／２２２）。发现随胎龄增加巢状及弥散状感染增多，ＣＭＶ往往先侵入绒毛霍夫保尔细胞。经电镜观察验证了ＰＡＰ法。结论免疫组化ＰＡＰ法简便、灵敏、有效。

Objective The purpose of this study is to set up a simple and quick diagnosis measurement of CMV infection in embryo, so as to diagnose the intrauterine infection CMV early.Methods PAP method and electron microscope were to diagnose the CMV infection.Results Two hundred and twenty-two of 608 cases of villi were (36.5%) infected by CMV. The focus of infection were classified into three types. The rate of spotty infection is 71%(158/ 222), the nestlike infection is 17%(37/ 222), the diffuse infection is 12%(27/ 222). The longer the pregnancy is the more nestlike infection and the diffuse infection were observed. We found CMV often intruded into Hofbauer cell first. The electron microscope observation was used to evaluate the PAP method.Conclusion The PAP method is a simple, convenient, and effective measurement. It can be used as a method for mass detection of CMV intrauterine infection.