血管紧张素转化酶基因多态性与特发性肺纤维化相关性

An Association between Angiotensin Converting Enzyme Genetic Polymorphism and Idiopathic Pulmonary Fibrosis

目的探讨血管紧张素转化酶(ACE)基因多态性与特发性肺纤维化(IPF)患者的相关性;监测IPF患者血浆中血管紧张素转化酶(ACE)活性、血管紧张素Ⅱ(AngⅡ)水平的变化。方法应用PCR技术扩增ACE基因目的片段检测基因多态性;采用紫外吸收法测定ACE酶的活性;采用竞争放射免疫分析法测定AngⅡ水平。结果(1)IPF患者与正常对照组的D等位基因频率及DD基因型分布有显著性差异(P<0.05)。(2)IPF患者与正常对照组的血浆ACE活性与AngⅡ水平有显著性差异(P<0.05)。结论(1)ACE基因I/D多态性与IPF发病有关,DD基因型是肺纤维化发病的危险因素。(2)血循环中ACE活性和AngⅡ水平升高与肺纤维化病变有关,两者可以促进肺纤维化的发生、发展。

Objective Fist,the aim of this study was to test hypothesis of an association between the polymorphism of ACE gene and idiopathic pulmonary fibrosis.Second,investigate the activity of ACE and angiotensinⅡ（AngⅡ） in IPF patients serum.Methods The polymorphism of ACE gene was analyzed with polymerase chain reaction（PCR）.The activity of ACE were determinated in all subjects serum.The level of AngⅡwere determinated in all subjects serum.Results（1）D-allele frequencies and DD genotype frequencies were higher in IPF patients than those in control subjects（P0.05）.（2）The activity of ACE and the level of AngⅡin serum were higher in IPF patients than those in control subjects（P0.05）.Conclusion（1）The ACE gene polymorphism are associated with IPF,and DD genotype of ACE gene is a risk factor for IPF patients.（2）The activity of ACE and the level of AngⅡin serumare associated with IPF,and they can advance the development of fibrosis in IPF patients.