摘要
目的探讨丹参注射液对慢性胰腺炎大鼠血清中HA和LN的影响,从而验证丹参注射液对慢性胰腺炎的治疗作用。方法通过胰管内注射2%三硝基苯磺酸的方法建立大鼠慢性胰腺炎纤维化模型。50只雄性SD大鼠随机分成5组:正常组、模型组、丹参小剂量组(400mg/kg)、丹参中剂量组(800mg/kg)、丹参大剂量组(1600mg/kg)。4周后应用免疫组化法(ELLSA)检测大鼠血清中的HA和LN的表达情况,同时将胰腺组织行HE染色,观察病理形态学改变。结果模型组发生明显的胰腺纤维化,血清中的HA和LN的表达明显升高。各不同剂量干预组纤维化程度及HA和LN的表达均低于模型组。结论丹参注射液可抑制大鼠胰腺纤维化组织的形成,对慢性胰腺炎有一定的治疗作用。
Objective To investigate the therapeutic effect of Danshen injection on Chronic Pancreatitis of Rats.Methods The content of HA and LN were detected in pancreatic normal group,control group and interference group using enzyme immunoassay（ELISA）and colorimetric method,observation the change of histology in pancreatic tissue by HE coloretur.Results The content of HA and LN in blood serum raise up obviously,the content of HA and LN in interference group is lower than that in control group.Conclusion Danshen injection has the effect to improve the pancreatic fibrosis of Rats.
出处
《中外医疗》
2010年第11期23-24,共2页
China & Foreign Medical Treatment
