输精管结扎后家兔血浆睾酮、前列腺睾酮结合容量和雄激素受体mRNA的变化

Changes of Plasma Testosterone, Prostatic Combination Capacities of Testosterone and Prostatic Androgen Receptors mRNA in Rabbits After Vasectomy

输精管结扎是世界范围内广泛应用的避孕措施，但输精管结扎对前列腺AR的影响尚未见报道。本文以日本大耳白兔分成输精管结扎组和假手术组，分别应用放免分析法、放射配体结合法和斑点印迹法对比观察了两组动物血浆睾酮水平、前列腺睾酮结合容量和前列腺ARmRNA的变化。结果发现输精管结扎后6月，血浆睾酮降低的同时。前列腺睾酮结合容量升高，ARmRNA水平降低。结扎后25月，血浆睾酮、前列腺睾酮结合容量、ARIRNA与对照组比较无明显差异。这说明输精管结扎早期血浆睾酮、前列除睾酮结合容量、ARmRNA的变化只是暂时性的。

As an effective and safe contraceptive method. vasectomy is worldwide accepted by many people, however. effects of vasectomy on prostatic androgen receptors (AR) are vague. Adult Japanese white rabbits (Department of Experimental Animal. NBUMS) were divided into vasectomy groups (VG) and sham operation groups (SOG). Contents of testosterone, prostatic combination capacities of testosterone and expression of prostatic AR mRNA were observed as radio-immunity assay. radioligand receptor assay and dot blot respectively. The results were follows: In VG6. contents of plasma testosterone decreased. while prostatic combination capacities of testosterone were significantly higher in VG6 than those in SOG6 (P ＜ 0. 05).Intensity of AR mRNA expression in SOG6 was stronger than that in VG6. There was no difference between VG25 and SOG25 in contents of testosterone. prostatic combination capacities of testosterone and intensity of AR mRNA expression. All of these showed that changes of contents of plasma testosterone. AR mRNA and combination capacities of testosterone in prostate were transient after vasectomy.