人造矿物纤维对细胞DNA损伤作用的体外研究

In vitro study on the DNA injuries induced by man made mineral fibers

目的比较研究１０种人造矿物纤维（ＭＭＭＦ）对人肺泡上皮细胞（Ａ５４９细胞）ＤＮＡ的损伤及修复作用。方法选用日本纤维状物质研究协会提供的１０种标准人造矿物纤维（ＪＦＭ），用单细胞凝胶电泳法，以Ａ５４９细胞作为靶细胞，测定了细胞ＤＮＡ链断裂、损伤后修复及ＤＮＡＤＮＡ链间交联。结果１０种ＪＦＭ纤维都可引起不同程度的ＤＮＡ链断裂、ＤＮＡＤＮＡ链间交联及抑制ＤＮＡ损伤后的修复功能。综合分析发现：微细玻璃纤维的遗传毒性最强，硅灰石的遗传毒性最低。它们的作用程度均低于温石棉。结论ＪＦＭ纤维均具有一定程度的体外遗传毒性，但均低于ＵＩＣＣ温石棉Ｂ。

Objective To study and compare DNA injuries and repair induced by 10 kinds of man made mineral fibers(MMMF) in human alveolar epithelial cell line(A549 cell).Methods Single cell gel electrophoresis(SCGE) assay was used to detect DNA strand breaks,DNA DNA interstrand crosslink and the ability of DNA repair when A549 cells were incubated with 10 kinds of JFM fibers and UICC chrysotile B respectively.Results All of 10 kinds of JFM fibers could induce DNA strand breaks,DNA DNA interstrand crosslink and inhibit the ability of DNA repair,but all of these effects were lower than that of chrysotile and were different among them.Conclusion All of 10 kinds of JFM fibers had genotoxicity with different degrees in vitro , but lower than that of chrysotile B.