L-精氨酸抑制高胆固醇饮食诱发的大鼠动脉血管细胞间粘附分子表达

Dietary L-arginine Attenuates Expression of Vascular Intercellular Adhesion Molecule in the Aorta of Hypercholesterolemic Rats

目的：观察L-精氨酸抗动脉粥样硬化的作用是否与其抑制大鼠动脉血管细胞间粘附分子（intercellularadhesionmolecule，ICAM－1）的表达有关。方法：Wistar大鼠24只，随机分为NC组（普通饲料及饮水），CC组（饲料中含4％胆固醇，1％胆酸），AC组（饲料中含4％胆固醇，1％胆酸，饮水中含3％L-精氨酸盐酸盐）；每组8只。8周后采血，处死Wistar大鼠取其主动脉作ICAM－1的免疫组织化学及信使核糖核酸（mRNA）NorthernBlot分析。结果：AC及CC组大鼠的血清胆固醇浓度无显著差异，但明显高于NC组。NC及CC组大鼠血清L-精氨酸浓度无明显差异，但明显低于AC组（P＜0．01）。L-精氨酸抑制高胆固醇膳食诱发的大鼠动脉ICAM－1表达。结论：L-精氨酸抑制动脉ICAM－1表达作用可能是其抑制高胆固醇膳食诱发的动脉粥样硬化的分子机制之一。

Objective:To determine whether the anti-atherogenic effect of L-arginine is due to an inhibition of vascular intercellularadhesion molecule expression in the aorta of hypercholesterolemic rats.Method:Twenty four male Wistar rats were randomized into three groups:Group NC was given normal diet (NC, n=8),group CC 4% cholesterol and 1% cholic acid diet (CC,n=8)and group AC 4% cholesterol and 1% cholic acid diet supplemented with 3% L-arginine in drinking water(n=8). After 8 weeks blood samples were collected for biochemical studies,and the aortas were harvested for Northern Blot and immunohistochemical studies.Results:The results showed: ①Serum cholesterol concentrations of rats of the AC and CC groups showed no significantdifference,but were significantly higher than those of the NC group. ②Serum L-arginine concentrations of the NC and CCgroups showed no significant difference, but were significantly lower than that of the AC group (p<0. of ). monetary Larginine supplementation (group AC ) significantly reduced the expression of vascular intercellular adhesion molecule(ICAM-1 ) both at the protein and messenger ribonucleic acid (mRNA)levels.Conclusion:Inhibitory effect of dietary supplementation of L-arginine on ICAM-1 expression may be one of the mechanisms of the antiatherosclerotic effect of L-arginine.