HPLC-ELSD法测定通迪胶囊中三七皂苷R_1和人参皂苷Rg_1、Rb_1的含量

HPLC-ELSD Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Tongdi Capsules

目的:采用HPLC-ELSD法测定通迪胶囊中三七皂苷R1和人参皂苷Rg1、Rb1的含量。方法:色谱柱DiamonsilC18(150mm×4.6mm,5μm),流动相:乙腈-水,梯度洗脱0～5min,乙腈20%～25%;5～20min,乙腈25%～45%;流速:1.0mL.min-1;柱温:25℃;漂移管温度:70℃;载气流速2.0L.min-1。结果:三七皂苷R1和人参皂苷Rg1、Rb1进样量分别在0.3～1.5μg(r=0.9997),1.5～7.5μg(r=0.9998)和1.5～7.5μg(r=0.9999)范围内呈良好的线性关系;平均回收率(n=6)分别为101.8%、102.1%、103.6%;RSD分别为1.6%、2.1%、1.8%。结论:HPLC-ELSO法结果准确,便于操作,可用于通迪胶囊的质量控制。

Objective：To develop the quantitative method of notoginsenoside R1,ginsenoside Rg1,and Rb1 in Tongdi capsules by HPLC -ELSD. Methods：The column was packed with a DiamonsilC18（150mm × 4. 6mm,5μm） stationary phase. The mobile phase consisted of acetonitrile -water,eluted in gradient mode（0 ～ 5min,acetonitrile 20% ～ 25% ;5 ～ 20min,acetonitrile 25% ～ 45% ） with flow rate of 1. 0ml.min -1. Column tem-perature was set at 25℃. The temperature of driftube was 70℃. Results：The linear ranges of notoginsenoside R1,ginsenoside Rg1 and Rb1were 0. 3 ～ 1. 5μg（r = 0. 9997）,1. 5 ～ 7. 5μg（r = 0. 9998）和 1. 5 ～ 7. 5μg（r = 0. 9999）,respectively; the average recoveries（n = 6） were 101. 8% 、102. 1% 、103. 6% ,with the RSD of 1. 6% 、2. 1% 、1. 8% respectively. Conclusion：The method is rapid,simple and accurate. It can be used for quality evaluation of Tongdi capsules.