大孔吸附树脂分离白鲜皮抗炎有效组分的实验研究

An Experimental Study on Anti-inflammatory Active Compounds of Cortex Dictamni Which was Separated by Macroporous Resin Column Chromatography

目的:探讨白鲜皮不同洗脱物的抗炎作用。方法:采用大孔吸附树脂柱色谱,分别以水、20%乙醇、50%乙醇、70%乙醇、95%乙醇为溶媒,对白鲜皮的95%乙醇提取物进行分离。通过二甲苯致小鼠耳廓肿胀模型,以吲哚美辛为阳性对照药物,考察各洗脱物的抗炎作用,筛选白鲜皮抗炎有效组分。结果:水洗脱物、20%乙醇洗脱物、50%乙醇洗脱物、70%乙醇洗脱物不具备抗炎活性;95%乙醇洗脱物有抑制二甲苯造成的非特异性耳廓肿胀的作用,与生理盐水比较,有显著性差异(P<0.05),与吲哚美辛作用相似。结论:95%乙醇洗脱物为白鲜皮的抗炎有效组分。

Objective：The anti-inflammatory effect of different elution compounds in Cortex Dictamni were ex-plored. Methods：95% ethanol extract of Cortex Dictamni was isolated by macroporous resin column chromatog-raphy （Water,20% ethanol,50% ethanol,70% ethanol,95% ethanol as solvent,respectively）. Theanti-inflammatory active constituents of Cortex Dictamni were selected with indome-thacin as a positive control drug by xylene-induced mouse ears edema model. Results：Water-eluted materials,20% ethanol eluted materials,50% ethanol eluted materials,70% ethanol eluted materials did not have anti-inflammatory activity but 95% ethanol eluted materials. Compared with saline,there was a significant differentce（P 0. 05）. However,there was no difference with indome-thacin. Conclusion：95% ethanol eluted materials in Cortex Dictamni had anti -inflammatory effect.