骨髓间质干细胞与肝癌细胞之间的作用及临床意义

The Effct and Clinical Significance of Co-cultured Liver Cancer Cells and Bone Marrow-derived Mesenchymal Stem Cells

目的研究肝癌细胞HepG2与大鼠骨髓间质干细胞(BMSCs)共培养后二者生物学行为的变化及其临床意义。方法分离SD大鼠BMSCs,经过鉴定后进行体外培养。利用Transwell小室将BMSCs与HepG2建立共培养模型,通过Transwell迁移实验、四甲基偶氮唑蓝(MTT)法和TUNEL法检测BMSCs和HepG2的生物学行为变化。结果HepG2能够促进BMSCs增殖,并对BMSCs具有明显的趋化作用。BMSCs、HepG2单独培养组和经过共培养后的BM-SCs和HepG2组所测得的细胞凋亡率分别为(5.8±0.3)%、(6.0±0.2)%、(6.3±0.2)%和(6.6±0.3)%,差异无统计学意义(F=13,P>0.05)。结论肝癌细胞HepG2对BMSCs具有明显的促增殖和趋化作用,利用携带细胞毒性药物或者抑癌基因的BMSCs向肝癌部位的趋化作用,有助于提高肝癌治疗的特异性和减少全身毒副作用。

Objective To investigate the biological behavior and its clinical significance of HepG2 and BMSCs after co-culture.Methods BMSCs were isolated and harvested from the bone marrow of SD-rats.BMSCs and HepG2 were co-cultured by transwell inserts in which the two chambers were separated by a semipermeable membrane.To investigate the biological behavior of them by transwell,MTT,and TUNEL.Results HepG2 could promote the progression of BMSCs significantly and initiate chemotaxis,but could not affect apoptosis.Conclusion If the engineered BMSCs which deliver cytotoxic drugs or express tumor suppressor gene could home to the liver tumor site,it may improve the specificity of liver cancer therapy and reduce the systemic toxicity.