海洋中药干贝的高效液相色谱指纹图谱研究

Study on HPLC Fingerprint of Chinese Marine Medicine Dried Scallop

目的建立海洋中药干贝高效液相色谱(HPLC)指纹图谱分析方法 ,为其质量控制和药材鉴别提供依据。方法采用反相Alltima-C18柱,水(A)和乙腈(B)为流动相,梯度洗脱,流速为0.8ml/min,检测波长210nm,柱温20℃,进样体积30μl;采用高效液相色谱二极管阵列检测器-质谱联用技术(HPLC-DAD-MS)对其特征峰进行快速分析;采用相似度计算软件用于10批干贝药材的相似度评价。结果初步建立了干贝药材的HPLC指纹图谱,10批干贝药材指纹图谱有9个共有峰,相似度均在0.85以上;HPLC-DAD-MS分析获得的4个特征峰的紫外光谱和质谱信息,可用于干贝药材指纹图谱特征峰以及共有峰的准确确定。结论该方法准确、简单、稳定性好,为干贝药材的真伪鉴别及质量控制提供了科学依据。

Objective To establish a chromatographic fingerprint of Dried Scallop,a kind of Chinese marine medicine,by high performance liquid chromatography （HPLC）,and apply for quality assessment and control of medicine materials of Dried Scallop.Methods Separation was performed on an Alltima-C18 （250 mm × 4.6 mm,5μm）,with mobile phase consisting of water （A） and acetonitrile （B） with gradient elution at the flow rate of 0.8 ml/min.The UV detection wavelength was 210 nm,column temperature was 20℃.Characteristic peaks of the fingerprint could be rapidly analyzed by high performance liquid chromatography-mass spectrometry （HPLC-MS）,and the software of similarity analysis was used to calculate the similarity of all the Dried Scallop samples.Results All samples tested contained the same nine peaks,and the similarities of 10 batches of Dried Scallop were more than 0.85.MS and UV spectra of the 4 characteristic peaks obtained using HPLC-MS could be used to accurately confirm the characteristic and common peaks in fingerprint of Dried Scallop.Conclusion This method is simple,accurate and stable,and can provide scientific reference for the identification and quality control of Dried Scallop.