粉防己碱对脂多糖致小鼠的痛觉增敏的影响及机制

Studies on Analgesic Effect and Mechanism of Tetrandrine on Lipopolysaccharide-induced Hyperalgsia in Mice

目的通过建立脂多糖(lipopolysaccharide,LPS)诱导痛觉增敏模型,以对乙酰氨基酚作为阳性药物,观察粉防己碱(Tetrandrine,Tet)对LPS致痛觉增敏小鼠的镇痛效应,进一步研究Tet镇痛与IL-6和TNF-α的关系。方法随机将小鼠分为空白组、模型组、对乙酰氨基酚组、Tet5,15,45mg/kg6组,通过腹腔注射LPS100μg/kg建立痛阈增敏疼痛模型,采用醋酸扭体法和热板法观察Tet的镇痛效应;ELISA法检测血浆中IL-6、TNF-α的含量变化。结果 Tet5,15,45mg/kg各组明显减少醋酸扭体次数,提高小鼠痛阈。减少血浆TNF-α含量、抑制IL-6的产生。结论 Tet具有镇痛活性,其作用机制可能是Tet抑制内毒素诱导小鼠血液中IL-6的产生、减少血浆TNF-α的含量。

Objective To investigate the effects of tetrandrine on LPS-induced hyperalgsia in Kunming mice and its possible mechanisms.Methods Mice were divided randomly into normal group,model group,acetaminophen group and Tet 5,15,45 mg·kg^-1 groups,hyperalgsia was induced by intraperitoneal administration of LPS 100 μg kg·kg^-1 in mice.The analgesic effect of Tet was assessed by the hot-plate stimulation and the writhing test induced by acetic acid,the contents of IL-6 and TNF-α in plasma were test by ELISA.Results Tetrandrine doses of 5,15,45 mg·kg^-1 significantly reduced the number of writhing response,increased pain threshold and decreased the contents of IL-6 and TNF-α.Conclusion Tetrandrine has analgesic effect and its effect may be related to decreasing the contents of IL-6 and TNF-α.