葛根总黄酮提取物的含量测定研究

Study on Determination of Pueraria Flavonoids in Extracts of Pueraria labta (Willd.) Ohwi

目的测定葛根提取物中有效成分葛根素及总黄酮的含量。方法采用高效液相色谱法对其中的葛根素进行含量测定;采用紫外分光光度法对其中的总黄酮进行含量测定。结果葛根素含量在0.017536～1.7536μg范围时与峰面积呈良好线性关系(r=0.99997),加样回收率为99.50%,RSD为1.36%;总黄酮的线性范围为2.192～10.92μg.ml-1(r=0.99955),加样回收率为100.69%,RSD为1.29%。结论所建立的分析方法简便、准确、可靠,重复性好,可用于葛根提取物中葛根素和总黄酮的测定。

Objective To determine the contents of puerarin and total flavonoids in extracts of Pueraria labta （Willd.） Ohwi.Methods HPLC was established to determine puerarin,and UV was established to determine total flavonoids in extracts of pueranva Labta.Results The results of HPLC quantitative analysis showed that the linear range of puerarin was 0.017 536～1.753 6 μg（r=0.999 97）,and the recovery rate was 99.05%,RSD=1.36%;the results of UV quantitative analysis showed that the linear range of total flavonoids was 2.192～10.92 μg·ml^-1（r=0.999 55）,and the recovery rate was 100.69%,RSD=1.29%.Conclusion This method is convenient,accurate,reliable with good reproducibility,so it can be used to determine the contents of puerarin and total flavonoids in extracts of Pueraria labta （Willd.） Ohwi.