涂石英玻璃交联毛细管柱的研制

The Preparation of Silica Coated Cross-Linked Glass Capillary Column

交联毛细管柱是在引发剂的作用下,毛细管内表面与固定液发生化学交联,生成一均匀的液膜,因而能抗溶剂冲洗,提高热稳定性,是一种新柱型。通常都是用静态法进行交联,构成所谓涂壁开管柱。此法制得的柱子较短,费时多,且液膜(d_f)较薄,

UItra-long silica coated and cross-linked glass capillary columns with SE-31.SE-54 and PEG-20M as stationary phases have been prepared. The silica suspension is coated on glass tubing before drawing. The stationary liquid is coated on capillary column by mercury plug method and the DCUP initiator by nitrogen pressure. The procedure is described in detail. The prepared columns are characterized by large inert surface and then liqaid film. Chromatograms showing good resolution and symmetry of peaks for polar compounds such as alcohols and free fatty acids are presented.