应用微生物群落分子指纹图谱指导筛选一株杨树灰斑病生防菌

Screen and Isolate an Antagonistic Strain against Coryneum populinum Bres.Guiding by Microbial Communities Fingerprint

杨树灰斑病是由杨棒盘孢菌(Coryneum populinum Bres.)引起的重要病害.运用拮抗微生物来防治杨树灰斑病已成为国际上的重点研究领域.本研究利用聚合酶链式反应-变性梯度凝胶电泳技术(PCR-DGGE)分析了同一苗圃中健康小黑杨、感病但未发病小黑杨及发病小黑杨叶际的细菌微生物群落,在健康小黑杨及感病但未发病小黑杨叶际发现一株优势菌YY-1.该菌在发病小黑杨叶际未检测到.利用地高辛标记探针指示,获得YY-1的纯培养并通过16SrDNA测序,分子鉴定该菌为蜡状芽孢杆菌(Bacillus cereus).对峙实验结果显示,该菌对杨树灰斑病病原菌杨棒盘孢菌具有较强的拮抗作用,可作为杨树灰斑病的生防菌.

Gray leaf spot of poplar caused by Coryneum populinum Bres.is an important plant disease.The use of antagonistic microorganisms to control the disease has become an international focus of research.Bacterial communities on healthy,susceptible and diseased poplar leaves in the same nursery are analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）.A predominant bacterium YY-1 is found.YY-1 is not detected on the poplar leaves which catch the gray leaf spot and identified as Bacillus Cereusthrough 16SrDNA sequencing.It has a strong antagonistic effect against Coryneum populinum bres.and can be used as a potential biocontrol agent to leaf gray spot of poplar.