摘要
目的建立香柏Sabina pingii(Cheng ex Ferre)var.wilsonii(Rehd.)Cheng et L.K.Fu总黄酮含量测定的方法.方法利用紫外分光光度法,用槲皮苷作对照品,测定香柏中总黄酮的含量.结果该方法重复性试验中RSD=0.92%,精密度试验中RSD=1.00%,回收率平均值为105.4%,三批香柏枝叶中总黄酮的含量平均为4.14%.结论该含量测定方法简便,准确,可用于香柏总黄酮的含量测定.
Objective:A method for determination of total flavones in Sabina pingii var.wilsonii is set up.Methods: Contents of total flavones are measured by Using UV spectrophotometry and quercitin is used as reference substance.Results: The RSD of repeatability of the experiments is 0.92%;the RSD of the precision of the experiment is 1.00%;Recovery is 105.4%,and the total flavones in the plant are assayed as 4.14%.Conclusion: The experimental method is convenient and accurate,and could be used for determination of total flavones in Sabina pingii.
出处
《西南民族大学学报(自然科学版)》
CAS
2010年第3期428-430,共3页
Journal of Southwest Minzu University(Natural Science Edition)
关键词
槲皮苷
紫外分光光度法
总黄酮
Quercetin
UV spectrophotometry
total flavone
contents determination