镉对小鼠卵巢颗粒细胞DNA损伤及MDA、SOD影响的初步研究

EFFECT OF CADMIUM ON DAN DAMAGE AND MDA AND SOD IN MOUSE OVARIES GRANULE CELLS

目的为探讨镉对雌性生殖机能的毒性影响。方法取小鼠卵巢颗粒细胞运用单细胞凝胶电泳技术检测10、20和40mg/kg体重的氯化镉攻毒48h后颗粒细胞DNA的损伤情况,并测定其MDA的含量和SOD的活性。结果镉对卵巢颗粒细胞DNA产生损伤作用,受损程度随镉剂量而升高,且MDA的含量升高,SOD的活性下降,具有明显的剂量效应关系,结论镉对卵巢颗粒细胞有毒性作用,能够损伤细胞DNA。

Objective： To study the toxicity of Cadmium on female reproduction function. Methods： The DNA damage of different doses of cadmium chloride were determined after 48h by single-cell gelelectrophoresis（SCGE） method, and the change of MDA and SOD were also determined. Results： The results showed that cadmium could induce the DNA damage in granule cells, increased the content of MDA, and decreased the SOD activity in a dose-dependent manner. Conclusion： Cadmium has toxicity on ovaries granule cells, could cause DNA damage.