由液相色谱保留值变化规律式进行固定相、流动相推荐和色谱峰定性

Selection of Mobile and Stationary Phases as well as Peak Identification Based on the FundamentalElution Equation in RP-HPLC

本文应用统计热力学导出的保留值变化规律式以及式中参数与溶质分子结构参数的关系,建立了反相色谱流动相和固定相的选择规则,并应用文献中的结果对这些规则进行了初步验证。本文还从分子结构和分子作用力出发,研究了各类组分用保留值变比规律式中参数与分子结构参数的定量关系进行色谱峰保留值定性的可行性。

In this paper,the rales for selection of mobile phase and stationary phase are generalized on the fundamental olution equation and quantitative relationship between parameters in fundamental elution equation and molecular structure parameters.These rules have been confirmed by the experimental results published in literatures.On the other hand,the possible ways to identify chromatographic peaks in wide range of mobile phase composition from the viewpoint of the molecular interaction between molecules of solute and molecules of each phase have also been inves-tigated