Exfoliazone通过诱导细胞凋亡抑制HepG2细胞体外增殖

Exfoliazone Inhibits the Growth of HepG2 Cells by Inducing Apoptosis

探讨Exfoliazone对肝癌细胞HepG2增殖及凋亡的影响.采用细胞培养技术,用不同浓度的药物在一定的时间内处理细胞株,应用MTT比色法检测Exfoliazone对HepG2细胞体外生长的抑制作用;4′,6-二脒基-2-苯基吲哚(DAPI)荧光染料染色,显示凋亡细胞形态;用Western-Blotting检测聚腺苷二磷酸核糖聚合酶(PARP)蛋白的表达;Annexin V/PI双染检测Exfoliazone对HepG2细胞凋亡的影响.Exfoliazone可抑制HepG2细胞的生长,并呈现剂量和时间的抑制,在药物处理24、48和72 h后,半数细胞抑制浓度(IC50)分别为35、16和8μmol/L.进一步研究表明,Exfoliazone可诱导PARP切割,细胞出现核质浓集和凋亡小体等典型的凋亡形态学特征,Annexin V/PI染色证实Exfoliazone能诱导HepG2细胞凋亡,并且以早期凋亡为主.Exfoliazone可能通过诱导HepG2细胞凋亡抑制癌细胞的生长.

This study is to investigate the anti-proliferation effect of Exfoliazone on liver cancer cell line HepG2 by inducing apoptosis.The test of anti-proliferation effect was performed by MTT assay,apoptotic cells were identified by DAPI staining and AnnexinV/PI staining.The expression of the poly ADP-ribose polymerase protein and its activated fragment were detected by Western-Blotting assays.Exfoliazone treatment significantly inhibited the growth of HepG2 cells in a time and dose-dependent manner.The IC50 for Exfoliazone was about 35,16 and 8 μmol/L after treatment for 24,48 and 72 h,respectively.Exfoliazone treatment could greatly induce apoptosis of HepG2 as indicated by DAPI staining,PARP cleavage and AnnexinV/PI staining.Exfoliazone inhibits the growth of HepG2 cells by inducing apoptosis.