水稻抽穗期QTLs的检测及上位性和环境互作效应

Mapping QTLs with epistatic effect and QTL×environmental interaction effect of heading date in rice

利用所构建的Lemont×特青重组自交系（RIL）,采用混合线性模型和复合区间作图法,对不同季节获得的水稻抽穗期QTL进行定位及上位性和环境互作效应分析.检测到3个控制抽穗期的QTL,分别位于第3、7和11号染色体上,共解释18.86%的遗传变异,单个QTL的表型贡献率为2.95%-10.56%,其中qHD7-1与环境存在显著互作,贡献率为2.18%;另检测到9对具有上位性效应的互作位点.

A population of 284 recombinant inbred lines （RIL） from the indica-japonica rice cross Lemont×Teqing was used to analyze the main effects, epistatic effects of the QTLs, and QTL by environmental interactions（QEs） controlling heading date（HD）. Three QTLs with additive effects for HD were mapped in the chromosome 3, 7 and 11, which accounted for 18.86% of the phenotypie variation. The percentage of phenotypic variation was explained by individual additive QTLs ranged from 2.95% to 10.56%. QTL×environmental interaction was detected on qHD7-1, which explained the phenotypic variation of HD with 2.18%. Epistasis analysis indicated that nine pairs of epistasis were detected for heading date. The results indicated that some QTLs were environmentally sensitive and epistasis was important genetic basis for heading date. The information obtained in this study should be very useful for manipulating the QTLs for heading date by molecular MAS.