FHIT在胃癌中的甲基化表达及其与幽门螺杆菌的关系

Methylation of FHIT gene in gastric cancer and its association with helicobacter pylori

目的检测胃癌(GC)组织中FHIT基因的甲基化表达及其与幽门螺杆菌(H.pylori)感染的关系。方法应用甲基化特异性PCR(MSP)检测74例GC组织中脆性组氨酸三联体(FHIT)基因的甲基化状态,并采用PCR检测上述标本中H.pylori感染情况。结果 GC组织中FHIT基因甲基化阳性率为51.4%,且甲基化与GC患者的年龄、性别、Lauren分型、Borrmann分型、淋巴结转移、浸润程度以及TNM分期无关(P>0.05);GC组织中H.pylori阳性率为52.7%,H.pylori感染与FHIT基因甲基化存在明显正相关(P<0.05,r=0.85)。结论 FHIT基因甲基化修饰在GC发生发展中起重要作用,且可能参与H.pylori感染致癌的病理途径。

Objective To investigate aberrant methylation of fragile histidine triad （FHIT） gene and to elucidate its ralationship with helicobacter pylori（H.pylor）infection in gastric cancer （GC）.Methods Methylation of FHIT gene was detected with methylation specific PCR （MSP） in cancer tissues from 74 patients with primary GC.Polymerase chain reaction （PCR） was used to amplify the ureB gene of H.pylori.Results Methylation analysis showed hypermethylation of FHIT gene in 51.4% cancer tissues.Methylation expression of FHIT gene in GC were irrelevant to age and gender of patients,Lauren’s classification,Borrmann’s classification,lymphatic metastasis,the depth of tumor invasion and TNM stage of tumor.H.pylori was found in 52.7% of GC tissues,there was positive correlation between FHIT gene methylation and H.pylori infection （P0.05,r=0.85）.Conclusion Methylation of FHIT gene may play critical roles in the pathogenesis and progression of GC,and it may be involved in the pathological pathways of H.pylori infection in GC.