摘要
目的 探讨三聚氰胺对孕鼠部分脏器及胎盘功能的危害.方法 40只Wistar孕鼠随机分为对照组(给予等量植物油)和3个实验组(给予三聚氰胺混配植物油,剂量从低到高分别为400mg/kg、800mg/kg、1 200mg/kg),每组10只,在其妊娠第6~19天连续灌胃,观察其体重变化及异常症状,妊娠第20天处死孕鼠,计算心脏、肝脏、肾脏、脾脏、肺脏、膀胱、子宫和卵巢组织的脏器系数及卵巢黄体数,观察卵巢、肾脏、肝脏、膀胱组织的病理改变,并测定血清孕酮、胎盘生乳素及雌三醇的浓度.结果 ①高剂量组出现不同程度的泌尿生殖系统中毒症状,体重下降较其它组明显.高、中剂量组均可见肾脏、卵巢结石或结晶形成,肝脏和膀胱轻度炎症反应,对照组和低剂量组的脏器均未发现上述改变;②高剂量组与对照组之间的脏器系数比较肾脏(P〈0.001)、卵巢(P〈0.01)、子宫(P〈0.05)、肝脏(P〈0.001)均有显著性差异,其余脏器系数各组比较无显著性差异(P〉0.05).高剂量组与对照组之间的黄体计数(P〈0.001)、孕酮值(P〈0.001)、胎盘生乳素值(P〈0.001)比较均有显著性差异.结论 三聚氰胺染毒诱发Wistar孕鼠肾脏、卵巢结石或结晶形成,造成组织损伤.此外可引起胎盘激素分泌异常,对胎盘功能及子代有潜在的危害.
Objective To investigate harms of melamine to part of organs and placental functions of pregnant rats. Methods 40 Wistar pregnant rats were randomly divided into control group ( n = 10, the rats were given equal amount of vegetable oil ) and melamine experimental group ( n = 30, the rats were given 400mg/kg, 800mg/kg and 1 200mg/kg of melamine-mixed oils respectively in each subgroups), The rats were mated in ratio of male: female = 2:1. From the 6th to the 19th days of pregnancy, the rats in the control group were continously fed with vegetable oil and those rats in the melamine experimental group were continously fed with 400mg/kg, 800mg/kg and 1200mg/kg of melamine respectively. The changes in body weight and abnormal symptoms of the pregnant rats in the two groups were observed. At the 20th days of gestation, the pregnant rats were sacrificed and the organ co-efficients ( organ relative weights ) of heart, liver, kidney, spleen, lung, bladder, uterus and ovary, and number of ovarian corpus luteum were caculated. The pathological changes in tissues of ovary, kidney, liver and bladder of the dead pregnant rats were observed. The serum levels of progesterone ( P ) , placental lactogen and estriol (E3 ) were detected by using radioimmunoassay method. Results (1) The rats in the high-dose melamine subgroup presented with different degrees of poisoning symptoms in urinary and reproductive systems, and the weight loss was also more significant than other subgroups and the control group. The lithiasis or crystal formation in kidney and ovary and mild inflammatory response in liver and bladder were found in the high and middle doses of melamine subgroups. While these changes were not found in the control group and low melamine dose subgroup; (2) In organ eo-efficients of kidney (P 〈0. 001 ), ovary(P 〈0. 01 ), uterus(P 〈0.05) and liver(P 〈 0. 001 ), there were significant differences between the high melamine dose group and the control group, while in organ co-efficients of other organs there were no significant differences ( all P 〉0. 05 ). Furthermore, in number of corpus luteum ( P 〈 0. 001 ) , serum levels of P (P 〈0. 001 ) and placental lactogen (P 〈0. 001 ), there were significant differences between the high melamine dose subgroup and the control group. Conclusion The exposure to melamine could induce lithiasis or crystal formation in kidney and ovary of Wistar pregnant rats, which may result in tissues injury. In addition, melamine may cause abnormalities in placental hormones secretion, which are potentially harmful to placental functions and the off-springs.
出处
《中国妇幼健康研究》
2010年第3期285-288,共4页
Chinese Journal of Woman and Child Health Research
关键词
三聚氰胺
毒性
结石
胎盘激素
melamine
toxicity
stone
placental hormone