EphA2蛋白在宫颈癌组织中的表达及意义

Expression of EphA2 in cervical cancer tissues and its significance

目的 探讨EphA2蛋白在宫颈癌组织中的表达及其意义.方法 收集宫颈鳞状细胞癌石蜡标本80例作为观察组,另收集宫颈上皮内瘤变石蜡标本20例及因良性病变行子宫切除术患者的正常宫颈组织10例作为对照组.采用多克隆抗体免疫组化SP染色法检测EphA2蛋白在宫颈癌组织中的表达水平.结果 EphA2蛋白在正常宫颈、宫颈上皮内瘤变、宫颈癌Ⅰb1和Ⅰb2期组织中表达的总体阳性率分别为20.0%、50.0%、80.0%、95.0%,总体阳性率比较差异有统计学意义（χ^2=31.476,P〈0.05）.观察组1（宫颈癌Ⅰb1期）和观察组2（宫颈癌Ⅰb2期）组织中EphA2蛋白表达的阳性率分别为80.0%、95.0%,经比较差异有统计学意义（χ^2=4.114,P〈0.05）.宫颈病变的进展与EphA2蛋白的表达强度之间呈线性相关（r=0.990,P=0.010）.高、中和低分化的宫颈癌组织中EphA2蛋白表达的阳性率分别为68.8%、90.6%和100.0%,经比较差异有统计学意义（χ^2=7.173,P〈0.05）;组织分化程度与EphA2蛋白阳性表达强度呈线性相关（r=0.977,P=0.016）.在有淋巴结转移和无淋巴结转移的宫颈癌组织中,EphA2蛋白的表达阳性率分别为100.0%和82.5%,经比较差异有统计学意义（χ^2=4.612,P〈0.05）.结论 EphA2蛋白的高表达与肿瘤大小、组织学分级、淋巴结转移等预后因素密切相关,其可望作为预测宫颈癌恶性程度及预后的分子指标.

Objective To study expression of EphA2 in cervical cancer tissues and its significance. Methods The paraffin specimens of 80 patients with cervical squamous cancer （as study group） and 20 patients with CIN（ as observation group） ,and normal cervical tissues of 10 patients who received hysterectomy due to benign cervical lesions （as the control group） were collected. The polyclonal antibody SP immunohistochemistry staining method was used to detect expression level of EphA2 in cervical cancer tissues. Results The positive rate of EphA2 in I b2 and I b1 cervical cancer tissues were significantly higher than that in CIN and normal cervical tissues （95.0%, 80.0% , 50.0%, 20.0% respectively; χ^2 =31. 476,P 〈 0.05 ）. The positive rate of EphA2 in stage I b2 cervical cancer tissues was significantly χ^2 higher than that in stage I bl cervical cancer tissues （95.0% vs 80.0% ; = 4. 114, P 〈 0.05 ）. There was a significant liner relation between progression of cervical cancer and expression intensity of EphA2 （ r = 0. 990, P = 0. 010 ）. The positive rate of EphA2 in pooly differentiated cervical cancer tissues and moderatedly differentiated cervical cancer tissues was significantly higher than that in highly differentiated cervical tissues （ 100.0%, 90.6% , 68.8% respectively ; χ^2 = 7. 242, P 〈 0.05 ） and there was a significant liner relation between differentiation degree of cervical cancer and expression intensity of EphA2 （ r = 0.977,P = 0.016）. The positive rate of EphA2 in cervical cancer tissues with lymphatic metastasis was significantly higher than that in cervical cancer tissues without lymphatic metastasis （ 100.0% vs 82.5% ;X^2 = 4. 612, P 〈 0.05） Conclusion There is a close relationships between expression of EphA2 and prognostic factors such as size of tumor, hystological grading and lymphatic metastasis. So EphA2 can be used as a molecular biological index to evaluate degree of malignacy and prognosis of cervical cancer.