佛山地区人乳头瘤病毒感染亚型分析

An investigation of epidemiology of HPV subtypes infection in Foshan city

目的 分析佛山地区人乳头瘤病毒感染亚型的分布情况.方法 对2008年11月～2009年10月在佛山市妇幼保健院宫颈疾病诊疗中心就诊的1 714例女性患者,采集宫颈脱落细胞,行反向点杂交人乳头瘤病毒基因分型检测和美国新柏氏细胞学检测.分析佛山地区人乳头瘤病毒亚型分布情况.结果 检测出570人（33.26%）感染了包括21种亚型的784个人乳头瘤病毒,其中HPV16亚型最高（21.95%）,其次为HPV52（16.70%）.16～25岁患者人乳头瘤病毒感染亚型以HPV6（19.78%）和HPV11（10.07%）为主;26～35岁患者人乳头瘤病毒感染亚型以HPV16（19.30%）和HPV6（17.09%）为主;36～45岁患者人乳头瘤病毒感染亚型以HPV16（18.92%）和HPV52（16.89%）为主;46岁以上患者人乳头瘤病毒感染亚型以HPV52（19.6%）为主.结论 反向点杂交基因芯片法可有效用于人乳头瘤病毒的检测和基因分型,佛山地区人乳头瘤病毒感染亚型分布具有明显的地域性.

Objective To investigate epidemiology of human papillomavirus （HPV） subtypes infection in Foshan city. Methods The cervical exfoliatied cells of 1 714 women who received gynecological examination in Cervical Disease Diagnosis and Treatment Center of Foshan Municipal Maternal and Child Health Hospital in a period from November, 2008 to October, 2009 were taken and analyzed for genotyping of HPV with array of reverse dot blot hybridization （RDB） and thin prep cytological test （TCT）. Results 570 women were infected by 784 HPVs, which belong to 21 subtypes including HPV subtypes 16, 18, 31, 33, 35, 39, 45, 51,52, 53, 56, 58, 59, 66, 68, 73, 83, 6, 11,42 and 43 with the positive HPV infection rate of 33.26% （570/1714）. Of which, the most common HPV genotypes were HPV-16（21.95% ）and HPV52（ 16.70% ）. The women aged 16 -25 years were mainly infected by genotypes of HPV6 （19.78%） and HPV11 （ 10.07% ）, those women aged 26 - 35 years old were mainly infected by genotypes of HPV16 （ 19.30% ） and HPV6 （ 17. 09% ）, those women aged 36 - 45 years were mainly infected by genotypcs of HPV16 （18.92% ）and HPV52 （ 16.89% ）and those women aged 〉45 years were mainly infected by genotype of HPV52（ 19.6% ）. Conclusion RDB technique is an effective method to detect and genotype HPV. The distribution of HPV genotypes infection in Foshan area is significantly regional.