假丝酵母99-125脂肪酶喷雾干燥工艺及催化性质的研究

Spray drying process for formulation of lipase from Candida sp. 99-125 and catalytic properties the powdered lipase

利用响应面法对假丝酵母脂肪酶喷雾干燥工艺条件进行优化,考察进口温度、雾化速度、保护剂含量对脂肪酶活力收率的影响。确定了最佳喷雾条件:保护剂为10～15 g/L的阿拉伯胶,进口温度115～120℃,雾化速度0.4 L/h,可得到收率最高为60.5%的脂肪酶酶粉。制得的固定化酶用于手性拆分(R,S)-1-苯乙醇,光学产率最高可达到53.6%;用于催化合成生物柴油,转化率最高可达到90.2%。在4、30℃下密封保存,半衰期可分别达到15个月、3个月。

The process condition for spray drying of Candida sp.99～125 lipase was optimized by a response surface methodology.The effects of the entrance temperature,the atomization speed,and additives were investigated.The optimized process parameters were as follows： the addition of the arabic gum was 1.0～1.5%,the entrance temperature was 115～120 ℃,and the atomization speed was 0.4 L/h,the highest yield of the enzyme activity could achieve 60.5%.The immobilized lipase was used for the resolution of rac-2-phenylethanol,the reaction obtained an e.e.53.6%;for the transesterification of oil with methanol,the conversion rate reached 90.2%.The half-life of spray-dried lipase powder under 4 and 30 ℃ were 15 and 3 months,respectively.