不同培养基对小球藻Chlorella zofingiensis生长和虾青素产量的影响

Effects of different culture media on the growth and production of astaxanthin by Chlorella zofingiensis

研究了小球藻Chlorella zofingiensis在3种不同培养基CZ-M1,Kuhl和KM1中培养时生物量和虾青素的产量。结果表明,Kuhl最利于小球藻的生长,比生长速率、最大细胞干重和得率最高,虾青素含量最低;KM1培养基最利于虾青素的积累。采用优化后的培养基KM1,添加葡萄糖诱导培养小球藻,可以获得8.99g·L-1的藻细胞干重,虾青素产量和含量分别达到20.1mg·L-1和2.24mg·g-1。

The biomass and astaxanthin yield of Chlorella zofingiensis cultivated in three different media including CZ-M1, Kuhl and KM1 were investigated. Results showed that Kuhl medium was best for growth as reflected by its highest values of specific growth rate, maximum cell dry weight concentration and cell growth yield on glucose comparing with the other media. However, its astaxanthin production was the lowest. KM1 exhibited the highest astaxanthin production both on a per dry cell weight and a per volume basis. Higher biomass （up to 8.99g＇L-1 cell dry weight concentration） and astaxanthin levels （up to 20.1mg.L-I and 2.24mg.g-1） in glucose-stressed Chlorella zofingiensis cells growing under low light in modified KM1 medium were obtained.