NaHSO_3对盐生杜氏藻生长和光合色素含量的影响

Effects of NaHSO_3 on the growth and contents of photosynthetic pigments in Dunaliella salina

为优化盐生杜氏藻营养盐配方、降低养殖成本、提高生物量及色素积累,研究了不同浓度的安全环保的植物循环光合磷酸化促进剂——NaHSO3(≤0.40mM·L-1)溶液对盐生杜氏藻生长和光合色素含量的影响。结果表明,NaHSO3可显著促进盐生杜氏藻生长,提高叶绿素a(Chl a)和b(Ch1 b)含量、叶绿素总量(Chl)、类胡萝卜素含量(Car)和Chl/Car;促进效应随浓度增加表现为先升后降,低浓度(<0.10mM·L-1)效应较好,以0.07mM·L-1为最佳。NaHSO3提高Chl b的效应大于Chl a,降低Chl a/Chl b比值,随浓度增加表现为先降后升,以0.07mM·L-1降低最大。本试验条件下,盐生杜氏藻生物量与光合色素含量间及各光合色素含量间呈显著或极显著相关,与生物量(Y,g·L-1)相关最大的是Chl b(XChl b,μg·ml-1),符合Y=0.284XChl b-0.883(R2=0.994**)(**表示Y与X之间在0.01水平上极显著相关,下同);其次为Chl(XChl,μg·ml-1),Y=0.100XChl-2.022(R2=0.993**),为生长动态的及时掌握提供了有效的间接衡量指标。

Bisulfite （NaHSO3） is a substance to accelerate plant cyclic photophosphorylation that can be used safely and envi- ronmentally friendly. In order to optimize the nutrients formula, decrease the production cost and improve the biomass and pigment accumulation ofDunaliella salina, the authors investigated the effects of different concentrations （0.00-0.40mM·L-1） of NaHSO3 on the biomass and photosynthetic pigment contents. The results showed that NaHSO3 could significantly improve the growth, contents of chlorophyll （Chl） a, Chl b, Chl （a＋b）, carotenoid （Car） and ratio of Chl to Car. The effects firstly in- creased and then decreased with the increase of concentration. Accelerating effects under low concentrations （〈0.10mM·L-l） were better than those under high concentrations （0.10-0.40mM·L-1）, and the maximum accelerating effects w.ere reaches at 0.07mM·L-1. NaHSO3 could increase Chl b content more than Chl a content, which caused the reduction of Chl a content rela- tive to Chl b content; the effects firstly decreased and then increased with the increase of concentration, reducing effects under low concentrations （〈0.1mM＇L-l） were better than those under high concentrations （0.10-0.40mM.L-I）, and the minimum reducing effect was achieved at 0.07mM-L-1. The correlations of biomass （Y） to contents of photosynthetic pigments and between contents of photosynthetic pigments were significant or markedly significant, with the highest correlation of Chl b （Xchl.b） to biomass （Y=O.284Xchl.b--0.883 （R2=0.994＊＊）） and the next of Chl to biomass （Y=0.100Xchl2.022 （R2=0.993＊＊））, which provided an indirect index to measure the growth ofDunaliella salina in every moment.