二十二碳六烯酸对胰腺癌细胞增殖和凋亡的影响

Effects of docosahexaenoic acid on the growth and apoptosis of human pancreatic cancer cell lines

目的观察二十二碳六烯酸(DHA)对人胰腺癌细胞株生长的抑制作用。方法将DHA与胰腺癌细胞混合培养,采用MTT、流式细胞技术分析细胞增殖、细胞凋亡、细胞周期以及凋亡相关蛋白Bcl-2含量。结果 50μg/mlDHA作用人胰腺癌细胞株后,Patu8988、SW1990增殖率24h为(46.89±5.99)、(46.03±7.69),48h为(35.92±2.91)、(25.70±5.60),肿瘤细胞增殖受到明显抑制(P<0.01)。同时细胞凋亡率明显升高,效应呈现时间-剂量依赖关系,72h后Patu8988、SW1990凋亡率分别为53.2%、13.7%。G0～G1期细胞比例增高,S期细胞比例下降。DHA作用24h后,Patu8988、SW1990Bcl-2蛋白表达率明显低于对照组(P<0.05)。结论 DHA能抑制胰腺癌细胞增殖,并通过下调Bcl-2在细胞中表达来诱导胰腺癌细胞凋亡。

Objective To investigate the effects of docosahexaenoic acid（DHA） on the growth and apoptosis of human pancreatic cancer cell lines.Methods Human pancreatic cancer cell lines Patu8988 and SW1990 were treated with DHA.The inhibition of cell proliferation was evaluated by MTT assay.Cell cycle,apoptosis induction and cyclooxygenase expression were detected by flow cytometry.Results After incubation of pancreatic cancer cells with DHA 50 μg/ml,cell proliferation rates of Patu8988 and SW1990 were （46.89±5.99） and （46.03±7.69） at 24 h and （35.92±2.91） and （25.70±5.60） at 48 h.The proliferation of tumor cells was suppressed obviously（P〈0.01）.Apoptosis was promoted in time-and dose-dependent manner.The cells at G0-G1 phase were increased,whereas those at S phase significantly decreased.After cultured with DHA for 24 h,the expressions of Bcl-2 protein for Patu8988 and SW1990 were significantly lower than those in control group（P〈0.05）.Conclusion DHA can inhibit the cell proliferation and induce apoptosis by down-regulating the expression of Bcl-2 protein.